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目的研究转化生长因子-β1(TGF-β1)、血管内皮生长因子(VEGF)和微血管密度(MVD)在结直肠癌侵袭转移中的作用及其相互关系。方法应用免疫组化技术检测52例结直肠癌手术标本TGF-β1、VEGF表达水平,分析以CD105标记的MVD计数和上述两种因子的表达及与结直肠癌临床病理因素的关系,Log-Rank时序检验MVD计数与生存时间的关系。结果52例结直肠癌中,以CD105标记的MVD均值为(11.27±1.55),MVD均值在Dukes分期C、D期组(17.86±2.07)和淋巴结有转移组(16.85±2.08)分别显著高于A、B期组(6.43±1.78)和淋巴结无转移组(7.78±1.93),差异均有高度统计学意义(均P>0.01),与患者性别、年龄、肿瘤部位、大小及分化程度均无关(均P>0.05)。TGF-β1阳性表达41例,VEGF阳性表达38例,分别与CD105标记的MVD计数明显相关(P<0.05)。本组病例3年生存率为39.1%,而MVD低于平均值组和高于平均值组的3年生存率分别为44.5%和30.1%,生存分析显示两组间差异有统计学意义(P<0.05)。结论以CD105标记的肿瘤新生血管计数可评估结直肠癌的侵袭转移,而TGF-β1、VEGF作为两种促血管生成因子,可能共同参与了肿瘤新生血管的形成。
Objective To investigate the role of TGF-β1, vascular endothelial growth factor (VEGF) and microvessel density (MVD) in the invasion and metastasis of colorectal cancer. Methods Immunohistochemistry was used to detect the expression of TGF-β1 and VEGF in 52 colorectal cancer specimens. The expression of CD105-MVD, the expression of these two factors and the clinicopathological factors of colorectal cancer were analyzed. Log-Rank Relationship between MVD count and survival time by time series test. Results The average MVD of CD105 markers in 52 cases of colorectal cancer was (11.27 ± 1.55). The average MVD in Dukes stage C, D group (17.86 ± 2.07) and lymph node metastasis group (16.85 ± 2.08) were significantly higher than The difference was statistically significant (all P> 0.01) between A and B group (6.43 ± 1.78) and lymph node without metastasis group (7.78 ± 1.93), which had no relation with gender, age, tumor location, size and differentiation (All P> 0.05). The positive expression of TGF-β1 in 41 cases and the positive expression of VEGF in 38 cases were significantly correlated with the counts of CD105-labeled MVD (P <0.05). The 3-year survival rate was 39.1% in this group, while the 3-year survival rates of MVD lower than the mean and higher than the mean were 44.5% and 30.1% respectively. The survival analysis showed that the difference between the two groups was statistically significant (P <0.05). Conclusion CD105-labeled tumor neovascularization can evaluate the invasion and metastasis of colorectal cancer. TGF-β1 and VEGF, as two pro-angiogenic factors, may participate in the formation of tumor neovascularization.