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从乙肝阳性人血清中克隆HBV含前s区表面抗原基因（PreSZ＋S），将其插入到杆状病毒转移质粒PVL1393中，并与昆虫病毒ACNPVDNA共转染Sf9昆虫细胞，筛选出重组病毒。用乙肝放免（RIA）试剂盒及EIAPreS2－Ag检测试剂盒检测重组病毒感染的Sf9细胞，表明细胞中有HBsAg及PreS2－Ag存在。将重组病毒感染的细胞免疫小鼠，在小鼠血清中能检测到anti-HBsAg及anti-PreS2抗体。
PreSZ + S was cloned from hepatitis B positive human serum and inserted into the baculovirus transfer plasmid PVL1393. The recombinant adenovirus was co-transfected with insect virus ACNPVDNA to Sf9 insect cells. The recombinant virus-infected Sf9 cells were detected by RIA kit and EIAPreS2-Ag assay kit, indicating that there are HBsAg and PreS2-Ag in the cells. Immunized mice with recombinant virus-infected cells detected anti-HBsAg and anti-PreS2 antibodies in mouse serum.