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为了建立蛔虫变应原致过敏性鼻炎的实验动物模型 ,将健康豚鼠 30只 ,随机分为致敏组 (n=2 0 )和对照组 (n=1 0 )。致敏组经腹腔、四肢皮下注射蛔虫变应原免疫 4周后 ,鼻腔雾化吸入蛔虫变应原 ;对照组以生理盐水代替变应原。结果致敏组 1 8只出现鼻庠、喷嚏、流清涕等过敏性鼻炎的临床症状 (评分 >5分 ) ,鼻腔分泌物涂片见大量嗜酸性粒细胞 ,以及鼻粘膜水肿、粘膜嗜酸性粒细胞和肥大细胞浸润等组织学变化 ,而对照组无上述变化 (P<0 .0 0 1 )。本实验成功建立的蛔虫变应原致过敏性鼻炎的实验动物模型 ,为过敏性鼻炎 ,特别是蛔虫变应原所致的过敏性鼻炎的诊断、治疗及研究工作提供了有力的方法及形态学依据。
In order to establish an animal model of allergic rhinitis induced by roundworm allergens, 30 healthy guinea pigs were randomly divided into sensitized group (n = 20) and control group (n = 10). In the sensitized group, the ascaris allergens were inhaled nasally after 4 weeks of immunization of the ascaris allergens in the abdominal cavity and extremities, and the control group was replaced by the saline. Results In the sensitized group, clinical manifestations of allergic rhinitis such as rhinitis, sneeze and runny nose were observed (score> 5), nasal secretion smear was found in a large number of eosinophils, nasal edema and mucosal eosinophilia The histological changes such as granulocyte and mast cell infiltration were not observed in the control group (P <0.01). The experimental animal model of Ascaris allergen-induced allergic rhinitis successfully established in this experiment provided a powerful method and morphology for the diagnosis, treatment and research work of allergic rhinitis, especially allergic rhinitis caused by Ascaris allergens in accordance with.