论文部分内容阅读
目的：探讨马钱子经炮制后毒性下降而生物效应增强的机制。方法：采用体外培养肿瘤细胞的方法，观察马钱子碱（ｂｒｕｃｉｎｅ）和异马钱子碱氮氧化物（ｉｓｏｂｒｕｃｉｎｅＮ－ｏｘｉｄｅ）对肿瘤细胞株ＨＥｐ－２生长抑制作用及形态损伤作用；并采用电镜观察心肌细胞。结果：异马钱子碱氮氧化物能明显地抑制肿瘤细胞生长，而马钱子碱则无明显此效应。其细胞的形态损伤作用与生长抑制作用相一致；异马钱子碱氮氧化物还能明显地抵消黄嘌呤—黄嘌呤氧化酶（ＸＸＯＤ）引起的破坏培养的心肌细胞肌丝和线粒体等超微结构的作用，而马钱子碱无此作用。结论：异马钱子碱氮氧化物具有抗肿瘤细胞生长和抗氧化作用，而马钱子碱却无此明显作用。
Objective: To explore the mechanism of the decrease of toxicities and the enhancement of biological effects of Curcumin. METHODS: In vitro culture of tumor cells was used to observe the effects of brucine and isobrucine N-oxide on growth inhibition and morphological damage of tumor cell line HEp-2. Observe the myocardial cells. Results: Isoniazid nitroxide can significantly inhibit the growth of tumor cells, but strychnine has no obvious effect. The morphological damage of the cells is consistent with the growth inhibitory effect; norrhylium oxynitrate can also significantly offset the damage of myocardium, mitochondria, etc., caused by xanthine-xanthine oxidase (X-XOD). The effect of ultrastructure, while brucine does not have this effect. CONCLUSION: Isoniazid nitrogen oxides have anti-tumor cell growth and anti-oxidation effects, while strychnine has no such effect.