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The amorphous I/Au composite nanofilms were prepared by low vacuum direct current sputtering (LVDCS) method. The optimized preparation technologies contain growth pressure, time, gaseous environment and annealing conditions. The maximum fluorescence emission (λemmax) of I/Au nanofilms was observed at wavelength of 375 nm, and the intensity of flu-orescence emission peak of annealed I/Au films was smaller than that of unannealed one due to fewer amorphous Au nanopar-ticles, caused by annealing treatment. In the UV-Vis absorption spectra, the intensity of UV-Vis absorption peak of annealed I/Au nanofilms is larger than that of the unannealed one. This work also developed a new way to grow I/Au composite fluores-cent thin films.