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目的建立灵敏快速检测人血浆中西洛他唑浓度的HPLC-MS/MS方法。方法采用色谱柱为Thermo Hy-purity C18(150 mm×2.1 mm,5μm,USA),流动相为乙腈(含0.1%甲酸)-甲醇=25∶75(v/v);流速为0.2mL.min-1;ESI+离子源,MRM进行离子方式监测。质谱参数:源电压3 500V,源温度100℃,去溶剂化温度350℃,锥孔气流速50 L.h-1,去溶剂化气流速350 L.h-1;血浆处理采用乙腈直接沉淀萃取方法。结果血浆中西洛他唑检测方法的线性范围为4.90~2 510 ng.mL-1,最低检出浓度为4.90 ng.mL-1,血浆中西洛他唑的方法回收率在80%~120%,日内RSD<10%,日间RSD<15%。结论本方法用于测定人血浆中西洛他唑的浓度,简单、快速、灵敏。
Objective To establish a sensitive and rapid HPLC-MS / MS method for the determination of cilostazol in human plasma. Methods The column was Thermo Hy-purity C18 (150 mm × 2.1 mm, 5 μm, USA). The mobile phase consisted of acetonitrile with 0.1% formic acid and methanol at 25:75 v / v. The flow rate was 0.2 mL · min -1; ESI + ion source, MRM for ion monitoring. Mass spectrometry parameters: source voltage 3500V, source temperature 100 ℃, desolvation temperature 350 ℃, cone flow rate 50 L · h-1, desolvation gas flow rate 350 L · h-1; Results The linear range of cilostazol in plasma was 4.90-2 510 ng · mL-1 with the lowest detectable concentration of 4.90 ng · mL-1 and the recovery of cilostazol in plasma was 80% -120% Days RSD <10%, daytime RSD <15%. Conclusion This method is suitable for the determination of cilostazol in human plasma and is simple, rapid and sensitive.