论文部分内容阅读
目的观察降铅Ⅰ号对低水平铅接触幼鼠血铅水平及学习记忆能力的影响。方法选用Wistar幼鼠60只,随机分成6组各10只,模型组、EDTA对照组和降铅Ⅰ号低、中、高剂量组饲喂0.2%醋酸铅去离子水制作铅接触模型,空白组饲喂普通去离子水。15 d后空白组取大鼠4只,其他组随机取2只测定血铅浓度,其他大鼠进行Y迷宫实验。大鼠铅接触模型制作成功后开始给药,EDTA对照组予依地酸钙钠(CaNa2)EDTA注射液10 mg.kg-1.d-1腹腔注射,降铅Ⅰ号低、中、高剂量组分别给予降铅Ⅰ号5,10,20 g.kg-1.d-1灌胃(给药之前稀释成相同容积),空白组和模型组予等量纯化水灌胃。各组均给药30 d后再检测血铅浓度并进行Y迷宫实验。结果给药30 d后,降铅Ⅰ号各剂量组血铅水平均较模型组显著下降(均P<0.05);Y迷宫实验显示,饲喂过醋酸铅的大鼠受电击次数明显多于空白组(P<0.01);降铅Ⅰ号各剂量组大鼠记忆保持率高于模型组(均P<0.05),其中降铅Ⅰ号中、高剂量组大鼠记忆保持率明显高于EDTA对照组(P<0.05)。结论降铅Ⅰ号能有效降低铅接触大鼠幼鼠的血铅水平,并能改善铅所致的学习障碍。
Objective To observe the effect of lowering lead I on blood lead levels and learning and memory ability of young rats exposed to low levels of lead. Methods Sixty Wistar rats were randomly divided into 6 groups and 10 rats. The model group, EDTA control group, and lead-lowering group I low, middle and high dose groups were fed 0.2% lead acetate deionized water to make lead exposure model. Feed regular deionized water. After 15 days, 4 rats in the blank group were randomly divided into 2 groups. Blood lead concentrations were measured randomly in other groups, and other rats were subjected to a Y-maze test. After the lead exposure model was successfully established in rats, the EDTA control group was given an intraperitoneal injection of calcium edetate (CaNa2) EDTA at a dose of 10 mg.kg-1.d-1, with low, medium, and high doses of lead-reducing I. The rats were given intragastric administration of 5, 10, and 20 g.kg-1.d-1 respectively (diluted to the same volume before administration). The blank group and model group were given equal volume of purified water. After 30 days of administration, blood lead concentrations were measured and Y-maze experiments were performed in each group. Results After 30 days of administration, the blood lead level of Jiangban I in each dose group was significantly lower than that of the model group (all P<0.05). The Y maze experiment showed that the rats fed with lead acetate had more shocks than the blank group. (P<0.01); Memory retention rate of rats in each group of Jiangban I was higher than that of the model group (all P<0.05). Among them, the memory retention rate of the middle and high dose group of Jiangban I was significantly higher than that of the EDTA control group. (P<0.05). Conclusion Jiangban I can effectively reduce blood lead levels in lead-exposed rat pups and improve learning disabilities caused by lead.