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本文应用ＥＬＩＳＡ双抗体夹心法定量检测血清肌红蛋白，３０名正常健康者均值为３５．４±１２μｇ／Ｌ；２５例急性心梗（ＡＭＩ）患者含量均超过正常人上限（＞７５μｇ／Ｌ），其异常率和临床符合率均达１００％，且血Ｍｂ含量与心梗面积密切有关。同时检测患者血清内心肌酶含量，结果显示ＣＫ、ＣＫ－ＭＢ、ＡＳＴ、α－ＨＢＤ和ＬＤＨ的临床符合率分别为５６％、４０％、８０％、７０％和８８％。ＡＭＩ患者用脲激酶进行溶栓治疗后，其血Ｍｂ含量也呈一过性增高。实验证明血清Ｍｂ含量增高是ＡＭＩ最早、最敏感的指标。采用ＥＬＩＳＡ法作血Ｍｂ定量具有特异性强，灵敏度好，精确度高，易于推广等优点，是ＡＭＩ实验室早期诊断的重要手段。
In this paper, ELISA double antibody sandwich method for the quantitative detection of serum myoglobin, 30 normal healthy average of 35.4 ± 12μg / L; 25 patients with acute myocardial infarction (AMI) patients exceeded the upper limit of normal (> 75μg / L) , The abnormal rate and clinical compliance rate reached 100%, and blood Mb content and myocardial infarction closely related. At the same time, the content of myocardium enzyme in serum was detected. The results showed that the clinical coincidence rates of CK, CK-MB, AST, α-HBD and LDH were 56%, 40%, 80%, 70% and 88% respectively. AMI patients with urea kinase thrombolytic therapy, the blood Mb levels also showed a transient increase. Experiments show that increased serum Mb is the earliest AMI most sensitive indicator. It is an important method for early diagnosis of AMI laboratory to use ELISA for blood Mb quantitatively with the advantages of strong specificity, good sensitivity, high precision and easy popularization.