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The purified thermophilic bacterium PS3 F1β10×His-tag is inserted into the FoF1-ATP synthases of chromatophores isolated from photosynthetic bacteria Rhodospirillum rubrum. The studies of biochemical properties of the hybrid chromatophores show that they have both protons-driving capability and photophosphorylation. The fluorescent actin filaments, as a marker of its orientation by video-microscopic experiment, are connected via MaleimidoC3-NTA to the reconstituted β10× His-tag of FoF1-ATP synthases.The clockwise rotation of FoF1-ATP synthases driven by light is observed directly when viewed from the Fo side to F1. This system should be valuable for further studying the coupling property of FoF1-ATP synthase.