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目的:探讨喉癌放疗前及放疗中细胞凋亡的变化及意义。方法:采用末端脱氧核苷酸转移酶介导的生物素化脱氧尿苷三磷酸缺口原位末端标记技术(TUNEL),检测27例喉癌患者放疗前及放疗中病变部活体组织癌细胞的凋亡率(AR)。结果:喉癌放疗前的AR为(21.0±0.7)%,放疗量分别为10、30及60Gy时的AR分别为(60.0±1.5)%、(42.0±1.3)%及(25.0±1.9)%,明显高于放疗前(P<0.05)。结论:检测喉癌患者活检癌细胞的AR有助于判断放射敏感性。
Objective: To investigate the changes and significance of apoptosis in laryngeal carcinoma before radiotherapy and radiotherapy. Methods: Terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick end labeling (TUNEL) was used to detect the apoptosis of cancer cells in 27 laryngeal cancer patients before radiotherapy and in radiotherapy Mortality (AR). Results: The AR of laryngeal carcinoma before radiotherapy was (21.0 ± 0.7)%, AR was (60.0 ± 1.5)%, (42.0 ± 1.3)% and (25.0 ± 1.9)% at radiotherapy doses of 10, 30 and 60 Gy respectively , Significantly higher than before radiotherapy (P <0.05). CONCLUSIONS: The detection of AR in biopsies of laryngeal cancer patients is helpful in determining radiosensitivity.