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文章介绍了一种低成本、快速、自动化的离心式微流控生化分析芯片。该芯片通过整合样本前处理和多生化指标检测,采用了多级微流道与虹吸微阀相结合的方式把样本的前处理、样本输送、样本与生化试剂反应等过程集成于一体,并通过比色检测获得各个生化检测项目的结果。实验结果显示芯片比色孔光程的精密度的变异系数在0.08%~0.52%,通过血糖结果的精密度评估的芯片对样本和稀释液定量以及两者混合的精密度变异系数为1.4%,同时使用芯片测定15个生化项目的日间和批内精密度均小于3.5%,这表明该离心式微流控生化分析芯片符合临床检测要求。
This article introduces a low-cost, fast, automated centrifugal microfluidic biochemical analysis chip. The chip through the integration of sample preparation and multi-biochemical detection, the use of multi-stage micro-flow path and siphon micro-valve combination of the sample pretreatment, sample delivery, sample and biochemical reagents and other processes integrated in one, and through Colorimetric Assay The results of each biochemical test are obtained. The experimental results showed that the coefficient of variation (CV) of the precision of the colorimetric aperture of the chip was between 0.08% and 0.52%. The precision of the glucose value was estimated by the chip, the sample and the diluent were quantified, and the coefficient of variation of the precision of the mixture was 1.4% The intra-day and intra-batch precision of 15 biochemical projects was less than 3.5%, which indicates that the centrifugal microfluidic biochemical analysis chip meets the clinical testing requirements.