论文部分内容阅读
人低密度脂蛋白用过氧化物酶标记(LDL-HRP)后,与成纤维细胞共同孵育,然后用肝素将结合在受体上的LDL-HRP洗脱下来,测定肝素洗液中LDL-HRP量和细胞数,并计算每个细胞表面的功能性受体数.结果表明,在普通培养液中,每个细胞表面平均有(7.5～8.2)×10~3个LDL受体;其活性受培养液中胆固醇浓度调节;胰岛素cGMP、可提高受体活性并促进细胞增殖;cAMP提高受体活性但抑制细胞增殖;放线菌素D抑制细胞增殖并降低受体活性.以LDL-HRP为探测剂,可对LDL受体进行超微结构形态学定位,根据酶反应产物的多少,也可以粗略地估计LDL受体活性.
Human LDL was labeled with peroxidase (LDL-HRP) and then incubated with fibroblasts. The LDL-HRP bound to the receptor was then eluted with heparin. LDL-HRP Amount and number of cells, and calculate the number of functional receptors on each cell surface.The results showed that there was an average of (7.5 ~ 8.2) × 10 ~ (3) LDL receptors on the surface of each cell in common culture medium, The concentration of cholesterol in the culture medium was adjusted, and insulin cGMP increased the activity of the receptor and promoted the proliferation of the cells. CAMP increased the activity of the receptor but inhibited the proliferation of the cells. Actinomycin D inhibited the proliferation and decreased the activity of the receptor. LDL-HRP Agent, LDL receptor ultrastructural localization of the receptor, according to the number of enzyme reaction products, but also a rough estimate of LDL receptor activity.