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为证实桑椹的抗肿瘤功能,以不同浓度桑椹汁处理结肠癌细胞株SW620,应用MTT法对比绘制生长曲线,调查SW620细胞的增殖情况,通过荧光定量PCR、SDS-PAGE检测SW620细胞凋亡相关基因p53的转录及蛋白质表达变化,分析桑椹汁对SW620细胞增殖、凋亡的影响机制。结果显示,桑椹汁对SW620细胞增殖的抑制作用随着桑椹汁浓度的升高而明显增强,以花青素质量浓度为6.267 3×10-3mg/mL的桑椹汁处理24 h后SW620细胞发生了以下变化:细胞的生长明显受到抑制,细胞脱落、变形与贴壁性下降,出现凋亡现象;细胞基因组DNA发生较明显的降解;荧光定量PCR检测细胞中p53基因的转录水平较对照组增加约6.5倍,SDS-PAGE检测p53蛋白的表达量也明显高于对照组。研究结果表明,桑椹汁对结肠癌细胞株SW620的增殖有抑制作用,其抑制机制可能是通过其活性物质诱导SW620细胞中p53基因上调表达导致细胞凋亡。
To confirm the antitumor function of mulberry leaves, the colon cancer cell line SW620 was treated with different concentrations of mulberry juice. The growth curve of SW620 cells was investigated by MTT assay. The proliferation of SW620 cells was investigated. The apoptosis-related genes of SW620 cells were detected by quantitative PCR and SDS-PAGE p53 transcription and protein expression changes, the impact of Mulberry juice on SW620 cell proliferation and apoptosis mechanism. The results showed that the inhibitory effect of mulberry juice on the proliferation of SW620 cells was significantly enhanced with the increase of mulberry juice concentration. SW620 cells were treated with mulberry juice of anthocyanin concentration 6.267 3 × 10-3mg / mL for 24 h The following changes were observed: cell growth was significantly inhibited, cell shedding, deformation and adhesion decreased, apoptosis occurred; cell genomic DNA was more significantly degraded; fluorescence quantitative PCR detection of p53 gene transcription level in cells increased compared with the control group 6.5-fold, the expression of p53 protein detected by SDS-PAGE was also significantly higher than the control group. The results showed that mulberry juice could inhibit the proliferation of colon cancer cell line SW620. The possible mechanism may be that apoptosis is induced by the upregulation of p53 gene in SW620 cells induced by its active substance.