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目的 应用经诱导和提取的小鼠肝癌Hep—A—22细胞株膜外抗原多肽在整体条件下观察对肿瘤的特异性免疫情况。方法 应用液闪仪检测免疫后小鼠胸腺和脾淋巴细胞对ConA的转化率及测量整体条件下肿瘤生长情况。结果 淋巴细胞对ConA发生转化率增高,同时诱导体内淋巴细胞特异性杀伤Hep—A—22细胞的活性明显增强,且几种组分的抗原多肽均能产生不同的诱导杀伤活性。经免疫后的小鼠肿瘤明显受到抑制。结论 提取的Hep—A—22肿瘤细胞膜外抗原多肽可作为肿瘤抗原进行有效的肿瘤特异性免疫,本研究为肿瘤特异性免疫治疗提供了新途径。
OBJECTIVE: To investigate the specific immune response to tumors using the extracellular membrane antigens of Hep-A-22 cells induced and extracted from mice. Methods The conversion rate of ConA in mouse thymus and spleen lymphocytes after immunization was measured by liquid scintillation detector and the tumor growth under the whole conditions was measured. Results The conversion rate of lymphocytes to ConA was increased, and the activity of lymphocyte specific killer Hep-A-22 cells was significantly enhanced in vivo, and antigenic peptides of several components could produce different induced killing activity. After immunization, the mouse tumor was significantly inhibited. Conclusion The extracted Hep-A-22 tumor cell membrane antigenic peptides can be used as tumor antigens for effective tumor-specific immunity. This study provides a new approach for tumor-specific immunotherapy.