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目的 :观察tranilast对转化生长因子 -β2 (Transforminggwowthfactor -β2 ,TGF -β2 )促进体外培养人眼小梁细胞胶原合成作用的影响。方法 :采用3 H -脯氨酸掺入及液体闪烁测量技术观察 0 μg·ml-1(对照组 )、 12 5 μg·ml-1、 2 5 μg·ml-1和 5 0 μg·ml-1tranilast对3 2ng·ml-1TGF -β2 促进体外培养人眼小梁细胞胶原合成作用的影响。结果 :12 5 μg·ml-1(q′ =4 2 6,P <0 0 5 )tranilast处理组3 H -脯氨酸掺入量为 62 0 3 3± 80 46,与对照组的 817 3 7± 12 4 2 1比较有显著差异 ;2 5 μg·ml-1(q′ =4 81,P <0 0 1)和 5 0 μg·ml-1(q′ =8 62 ,P <0 0 1)tranilast处理组3 H -脯氨酸掺入量为5 94 5 8± 88 13、 418 64± 67 90 ,与对照组比较有极显著差异 ;同时 ,3 H -脯氨酸掺入量随tranilast浓度增加而减少 ,呈现明显的量效关系。结论 :Tranilast明显抑制TGF -β2 对体外培养人眼小梁细胞胶原合成的促进作用。利用tranilast防治原发开角型青光眼的可能性值得作进一步研究。
OBJECTIVE: To observe the effect of tranilast on collagen synthesis induced by Transforming growth factor-β2 (TGF-β2) in human trabecular meshwork cells. Methods: 3 H - proline incorporation and liquid scintigraphy were used to observe the effects of 0 μg · ml-1 (control group), 125 μg · ml-1, 25 μg · ml-1 and 50 μg · ml- Effects of 1 transnilast on collagen synthesis induced by 3 2 ng · ml-1 TGF-β2 in cultured human eye-trabecular meshwork cells. Results: The amount of 3 H - proline incorporation in the tranilast treated group was 62 0 3 3 ± 80 46 with 12 5 μg · ml-1 (q ’= 426, P <0.05) 7 ± 12 4 2 1 (p <0 0); 2 5 μg · ml -1 (q ’= 4 81, P 0 01) and 50 μg · ml -1 1) The amount of 3 H - proline incorporation in tranilast treatment group was 5 94 5 8 ± 88 13 and 418 64 ± 67 90, respectively, which were significantly different from those in control group tranilast concentration increased and decreased, showing a significant dose-effect relationship. CONCLUSION: Tranilast significantly inhibits the promotion of TGF-β2 on collagen synthesis in cultured human trabecular meshwork cells. The use of tranilast prevention and treatment of primary open-angle glaucoma is worth further study.