用地衣红按常规法能将肝细胞中HBsAg染色,但退化性及坏死性肝细胞原浆亦可着色,从而给判定HBsAg带来困难。本文介绍的地衣红染色改良法,仅对肝细胞中HBsAg着色呈阳性,无假阳性,可获得稳定而满意的结果。其原理是:在作常规染色时,由于地衣红进入肝细胞蛋白质分子结构的间隙引起了同时着色,如切片先用氧化液,再用致敏液(硫酸铁铵或硝酸铀溶液)处理,则肝细胞蛋白质分子结构的间隙便被铁或铀离子充满,致使地衣红不能进入,故肝细胞原浆不被地衣红着色。本文指出,不同的HBsAg染料均能与蛋白质二硫基氧化形成的硫酸残基反应,从而提出了一种新的雷琐辛-复 Licorice red according to the conventional method will be able to HBsAg staining of liver cells, but degenerative and necrotic hepatocyte puree can also be colored, which makes it difficult to determine the HBsAg. This article describes the lichen red staining improved method, only the positive staining of HBsAg in liver cells, no false positive, stable and satisfactory results can be obtained. The principle is: in the conventional dyeing, due to lichen red into the gap between the molecular structure of liver cells caused by the simultaneous coloring of the protein, such as the first slice with the oxidation solution, then the sensitized solution (ammonium ferric sulfate or uranium nitrate solution), then Liver cell protein molecular structure of the gap will be filled with iron or uranium ions, resulting in lichen red can not enter, so the liver cell puree is not lichen red coloring. This article points out that different HBsAg dyes can react with the sulfate residues formed by the disulfide oxidation of proteins, and thus a new rezosin-complex