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目的探讨复方甘草酸苷对大鼠亚急性酒精性肝损伤的保护作用及其相关机制。方法将72只健康SD大鼠随机分为6组:空白组、模型组、复方甘草酸苷低剂量组、中剂量组、高剂量组和阳性对照药葵花护肝片组。采用白酒灌胃的方法建立大鼠亚急性酒精性肝损伤模型,在造模的同时灌胃复方甘草酸苷及葵花护肝片水溶液。连续灌胃1周,然后检测血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)及肝组织中超氧化物岐化酶(SOD)、丙二醛(MDA)水平;并对肝脏作组织病理学检查。结果与空白组比较,模型组大鼠血清ALT、AST活性和肝组织MDA含量显著增加(P<0.01);肝组织SOD活性显著降低(P<0.01)。与模型组比较,复方甘草酸苷高剂量组大鼠血清ALT活性水平显著降低(P<0.01);低、中、高剂量组血清AST活性和肝组织MDA含量降低(P<0.05);中、高剂量组大鼠肝组织SOD活性升高(P<0.05)。结果提示,复方甘草酸苷各剂量组能改善肝脏组织病理损伤。结论复方甘草酸苷对酒精所致大鼠亚急性肝损伤具有保护作用。
Objective To investigate the protective effect of compound glycyrrhizin on subacute alcoholic liver injury in rats and its related mechanism. Methods Seventy-two healthy SD rats were randomly divided into 6 groups: blank group, model group, compound glycyrrhizin low dose group, middle dose group, high dose group and positive control drug sunflower liver tablet group. A rat model of subacute alcoholic liver injury was established by gavage of liquor. At the same time, glycyrrhizin and sunflower liver tablet were instilled in the model. The rats in each group were given continuous gavage for 1 week. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and superoxide dismutase (SOD) and malondialdehyde (MDA) levels in liver tissue were detected. Histopathological examination . Results Compared with the blank group, the serum ALT and AST activities and the content of MDA in the liver tissue of the model group were significantly increased (P <0.01). The activity of SOD in the liver tissue was significantly decreased (P <0.01). Compared with the model group, serum ALT activity of compound glycyrrhizin high dose group was significantly lower (P <0.01); serum AST activity and liver MDA content of low, medium and high dose group were decreased (P <0.05) High-dose group rats liver SOD activity increased (P <0.05). The results suggest that compound glycyrrhizin in each dose group can improve liver tissue pathology. Conclusion Compound glycyrrhizin has a protective effect on alcohol induced sub-acute liver injury in rats.