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目的探讨血管紧张素原基因M235T多态性与糖尿病慢性肾脏疾病(CKD)的关系。方法选取健康对照(NC)者203名与T2DM患者311例(UAlb/Cr<30mg/g组158例,30mg/g≤UAlb/Cr<299mg/g组111例和UAlb/Cr≥300mg/g组42例),采用聚合酶链反应-限制性片断长度多态性技术进行M235T位点多态性检测。结果 NC组和T2DM组基因型分布MM、MT、TT分别为2.5%、29.6%、68.0%和2.6%、28.3%、69.1%,两组等位基因M、T频率分别为17.2%、82.8%和16.7%、83.3%。4组间基因型分布比较差异无统计学意义,等位基因频率比较差异无统计学意义。进一步按照性别比较,4组基因型分布比较差异无统计学意义,等位基因频率比较差异无统计学意义。结论血管紧张素原基因M235T多态性可能与CKD无相关性。
Objective To investigate the relationship between angiotensinogen gene M235T polymorphism and chronic kidney disease (CKD) in diabetes. Methods A total of 203 healthy controls (NC) and 311 T2DM patients (158 patients with UAlb / Cr <30mg / g, 111 patients with 30mg / g≤UAlb / Cr <299mg / g and UAlb / Cr≥300mg / g 42 cases). The polymorphism of M235T was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The genotype distributions of MM, MT and TT in NC group and T2DM group were 2.5%, 29.6%, 68.0% and 2.6%, 28.3% and 69.1%, respectively. The M and T allele frequencies of the two groups were 17.2% and 82.8% And 16.7% and 83.3% respectively. There was no significant difference in genotype distribution between the 4 groups, but no significant difference in allele frequencies. Further according to gender comparison, there was no significant difference in the distribution of genotypes between the four groups, and there was no significant difference in allele frequencies between the four genotypes. Conclusion There is no correlation between the M235T polymorphism of angiotensinogen gene and CKD.