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目的探讨ｐ５３蛋白、增殖细胞核抗原（ＰＣＮＡ）在肺癌中表达的特征、相关性及与ＤＮＡ含量和预后的关系。方法应用免疫组织化学方法检测原发性肺癌组织标本ｐ５３蛋白和ＰＣＮＡ的表达，并用图像分析技术测定ＤＮＡ含量。结果ｐ５３蛋白阳性表达组ＤＮＡ含量（８．９１Ｃ）和ＰＣＮＡ半定量分级（Ｉ～Ⅳ级例数为９，１３，１２，１０，下同）显著高于ｐ５３蛋白阴性组（６．６０ｃ）（１４，１５，７，２）在肺腺癌，半年内死亡组ｐ５３蛋白阳性表达率（１１／１５）和ＰＣＮＡ半定量分级（２，５，４，４）显著高于５年以上生存组（７／１９）（１０，６，１，２），且两者表达与淋巴结转移有显著关系；在肺鳞癌，半年内死亡组ＰＣＮＡ半定量分级（４，１，６，２）显著高于５年以上生存组（６，１４，７，０），而ｐ５３蛋白表达与患者预后关系不明显。结论ｐ５３蛋白、ＰＣＮＡ及ＤＮＡ含量间具有显著正相关关系，且ｐ５３蛋白、ＰＣＮＡ表达对于估测肺癌患者的预后有重要意义。
Objective To investigate the expression and correlation of p53 protein and proliferating cell nuclear antigen (PCNA) in lung cancer and their relationship with DNA content and prognosis. Methods Immunohistochemical method was used to detect the expression of p53 protein and PCNA in primary lung cancer tissue samples. DNA content was determined by image analysis technique. Results The p53 protein positive expression group DNA content (8.91C) and PCNA semi-quantitative grading (9, 13, 12, 10, respectively, the same number of cases of grade I to IV) were significantly higher than the p53 protein negative group (6.60c) ( 14,15,7,2) In lung adenocarcinoma, the positive expression rate of p53 protein (11/15) and PCNA semi-quantitative classification (2,5,4,4) were significantly higher in the death group than in the survival group of more than 5 years in the death group ( 7/19) (10,6,1,2), and there was a significant relationship between their expression and lymph node metastasis. In lung squamous cell carcinoma, semi-quantitative classification (4,1,6,2) of PCNA was significantly higher in the death group within half a year. Over 5 years survival group (6,14,7,0), but the relationship between p53 protein expression and prognosis was not obvious. Conclusion There is a significant positive correlation between p53 protein, PCNA and DNA content, and the expression of p53 protein and PCNA is important for estimating the prognosis of lung cancer patients.