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目的了解秦皇岛市水产品中副溶血弧菌菌群菌型分布,进一步对毒力基因和药敏进行检测,为预防副溶血弧菌引起的食源性疾病,并有效地指导临床合理用药提供依据。方法收集2005—2015年秦皇岛市水产品中分离出的副溶血弧菌440株,利用玻片法进行血清学试验,PCR方法对其携带的TDH和TRH基因扩增,并测定耐药情况。结果水产品中分离出的副溶血弧菌分为O1、O2、O3、O4、O5、O6、O10、O11,共8个群,优势群为O5群,血清型为O5∶K17;携带TDH毒力基因的有41株,携带TRH毒力基因的有3株,还有1株既携带TDH又携带TRH基因,占总数的百分比分别为9.3%、0.7%和0.2%;氨基糖苷类和喹诺酮类抗生素为抗感染首选。结论副溶血弧菌对多数抗菌药物存在不同程度的耐药,应加强水产品卫生监管,同时加大抗生素的使用管理,有针对性的选择抗菌药物。
Objective To understand the distribution of strains of Vibrio parahaemolyticus in aquatic products in Qinhuangdao and to further detect the virulence genes and drug susceptibility, to provide basis for preventing foodborne diseases caused by Vibrio parahaemolyticus and effectively guiding clinical rational drug use . Methods 440 isolates of Vibrio parahaemolyticus isolated from aquatic products in Qinhuangdao City during 2005-2015 were collected and serologically tested by slide method. The TDH and TRH genes amplified by PCR were amplified and the drug resistance was determined. Results Vibrio parahaemolyticus isolated from aquatic products was divided into 8 groups: O1, O2, O3, O4, O5, O6, O10 and O11. The dominant group was O5 and the serotype was O5: K17. 41 strains of TR gene, 3 strains of TRH virulence genes and 1 strain of TRH genes carrying TDH and TRH, accounting for 9.3%, 0.7% and 0.2% of the total respectively. Aminoglycosides and quinolones Antibiotics is the first choice for anti-infection. Conclusion Vibrio parahaemolyticus has resistance to most antibacterials to varying degrees. It is necessary to strengthen the hygiene supervision of aquatic products, increase the use and management of antibiotics, and select the antibacterials selectively.