论文部分内容阅读
目的探讨砷对红细胞红外光谱及红细胞的蛋白质二级结构的影响,寻找砷中毒的早期损伤指标。方法健康雄性SD大鼠32只,分为对照组及低、中、高砷染毒剂量组。通过自由饮水方式染毒2个月,砷浓度依次为0、80、400和2 000μg/l;处死动物,采集大鼠抗凝血液,进行红细胞参数及FT-IR检测;分析样品红外光谱的酰胺I带,得出红细胞中蛋白质二级结构的信息。结果砷染毒组与对照组比较,红细胞各项参数未发生明显改变(P>0.05);各组大鼠红细胞红外光谱主要包括Amide I、Amide II、C=C=N伸缩振动吸收峰及水的—OH吸收峰;砷染毒组大鼠红细胞中蛋白质的二级结构发生改变,以高剂量组的改变最明显(P<0.05);随染毒剂量的升高,α-螺旋的百分比逐渐降低。结论FT-IR测得的大鼠红细胞蛋白质的α-螺旋相对含量可作为砷中毒时机体损伤的一个指标。
Aim To investigate the effect of arsenic on erythrocyte infrared spectroscopy and erythrocyte protein secondary structure and to find the early damage index of arsenic poisoning. Methods Thirty-two male Sprague-Dawley rats were divided into control group and low, medium and high arsenic-exposed dose groups. Arsenic concentrations were 0, 80, 400 and 2 000 μg / l, respectively. Animals were sacrificed and the anticoagulant blood of rats was collected. The erythrocyte parameters and FT-IR were measured. The infra red I band, derived from the red blood cells in the secondary structure of protein information. Results Compared with control group, the parameters of erythrocytes in arsenite-treated group did not change significantly (P> 0.05). The infrared spectra of rat erythrocytes mainly included Amide I, Amide II, C = C = N stretching vibration absorption peak and water (P <0.05). With the increase of exposure dose, the percentage of α-helix gradually increased reduce. Conclusion The relative content of a-helix of rat erythrocyte protein measured by FT-IR can be used as an indicator of body damage in arsenic poisoning.