论文部分内容阅读
以水溶性碳化二亚胺法将甲胺磷（ＭＴＰ）分子偶联到牛血清白蛋白（ＢＳＡ）上，用此合成的免疫原复合物制备了特异性的兔抗甲胺磷多抗血清。抗血清的ＥＬＩＳＡ效价为１∶１２８００～１∶５１２００；抗血清与乙酰甲胺磷的交叉反应率为１０．５％，与供试的其它９种有机磷农药的交叉反应率均小于２．８％。用同样的方法合成了甲胺磷－卵清蛋白复合物作为包被抗原，建立了适于环境中甲胺磷残留分析的间接竞争ＥＬＩＳＡ工作程序，在明确了该方法的工作条件及其基本参数的基础上，用建立的ＥＬＩＳＡ方法测定了稻谷和小白菜叶片样品中的甲胺磷残留量，并与ＧＣ方法的测定结果进行了比较。结果表明：建立的ＥＬＩＳＡ方法对甲胺磷纯品的最低检测量为０．８５ｎｇ／ａｓｓａｙ，线性检测范围为０．０３２～５００μｇ／ｍＬ；在该范围内，标准曲线的批内和批间变异系数分别为３．７９％和４．４３％；稻谷和小白菜叶片样品中添加甲胺磷后的平均回收率分别９０．３％和９３．２％；在测定的３１份稻谷和４５份小白菜样品中，ＥＬＩＳＡ检测为阳性的样品百分率分别为１６．１％和４６７％，阳性样品中甲胺磷的残留量为０．０２４～７４．９６２ｍｇ／ｋｇ；ＥＬＩＳＡ测定结果与ＧＣ测定结果呈显著的线性?
Methamidophos (MTP) molecules were coupled to bovine serum albumin (BSA) by a water-soluble carbodiimide method, and specific rabbit anti-methamidophos polyclonal antibodies were prepared using the immunogenic complexes. The ELISA titer of the antisera was 1:12800 ~ 1: 51200; the cross-reactivity of antisera with acephate was 10.5%, and the cross-reactivity with other 9 organophosphorus pesticides tested was less than 2. 8%. Methamidophos-ovalbumin complex was synthesized in the same way as coating antigen, and an indirect competitive ELISA procedure suitable for the analysis of methamidophos in the environment was established. The working conditions and basic parameters The residues of methamidophos in rice and cabbage leaves were determined by the established ELISA method and compared with the results of GC method. The results showed that the minimum detection limit of established ELISA for methamidophos was 0.85ng / assay, and the linear range was 0.032-500μg / mL. Within this range, the intra- and inter-batch variation of the standard curve The coefficients were 3.79% and 4.43%, respectively. The average recoveries of methamidophos in rice and cabbage leaves samples were 90.3% and 93.2%, respectively. Among the 31 rice and 45 small In cabbage samples, the percentage of positive samples detected by ELISA was 16.1% and 46.7%, respectively. The residues of methamidophos in positive samples were 0.024-74.962 mg / kg. The results of ELISA and GC Was significant linear?