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研究表明,甘蓝型油菜(Brassica napus L.)花粉胚状体的诱导频率与培养材料的基因型、培养基蔗糖浓度、培养基附加成分、培养基状态、温度处理等因素有关。不同基因型材料的花粉胚状体诱导频率明显不同;培养基蔗糖浓度以10％为最佳;B_5培养基附加2,4-D 0.1mg/l和NAA0.1mg/l的培养效果最好;液体浅层静止漂浮培养优于固体培养;变温处理可以提高花粉胚状体诱导频率。本文也报道了五种不同的花粉胚状体类型与植株再生的研究。
The results showed that the induction frequencies of pollen embryoids in Brassica napus L. were related to the genotypes of cultivated materials, the sucrose concentration in the medium, the additional components of the medium, the state of the medium and the temperature treatment. The induction frequencies of pollen embryoid bodies of different genotypes were significantly different; the best sucrose concentration was 10%; the best culture effect was B5 medium supplemented with 2,4-D 0.1 mg / l and NAA 0.1 mg / l; The superficial static floating culture of liquid is better than that of solid culture. The temperature-induced treatment can increase the frequency of pollen embryoid induction. This paper also reports on five different pollen embryoid body types and plant regeneration.