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目的:比较中药壁虎鲜品与炮制品对小鼠H22肝癌细胞体内外生长抑制作用的强弱。方法:体外试验采用MTT法,检测壁虎提取液冻干粉对H22细胞的增殖抑制情况;体内试验采用小鼠肝癌H22移植瘤法,比较对实体肿瘤的抑制作用,并检测药物对小鼠胸腺指数及脾指数的影响。结果:在体外试验中,壁虎鲜品和炮制品对H22细胞的半数抑制率IC50分别为8.53、6.42 mg/ml;体内试验,鲜品和炮制品最大抑瘤率分别为53.0%和47.4%。炮制品各组的胸腺指数和脾指数均高于环磷酰胺(CTX)阳性对照组,鲜壁虎的中、高剂量组胸腺指数和低剂量组脾指数较阳性对照组高。结论:鲜品与炮制品均有抗肿瘤作用,但二者活性差异不显著;炮制品对胸腺和脾脏有生长刺激作用。
OBJECTIVE: To compare the strength and weakness of the in vitro and in vivo inhibition of H22 hepatocarcinoma cells induced by Gekko fresh goods and processed products. Methods: MTT assay was used in vitro to test the inhibitory effect of gecko extract freeze-dried powder on the proliferation of H22 cells. In vivo experiments were performed on the H22 tumor-bearing mouse hepatocarcinoma model to compare the inhibitory effect on the solid tumors. The thymus index And spleen index. Results: In vitro tests showed that the IC50 of HGC cells treated with fresh and processed gecko was 8.53 and 6.42 mg / ml, respectively. The in vivo tests showed that the maximum inhibition rates of fresh and processed gecko were 53.0% and 47.4%, respectively. The thymus index and spleen index in each group were higher than that in CTX positive control group. The index of thymus index and the spleen index of low and middle dose gecko group were higher than that of positive control group. Conclusion: Both fresh and processed products have anti-tumor effects, but the difference between them is insignificant. The processed products have growth-stimulating effects on thymus and spleen.