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对前期在苹果(Malus×domestica Borkh.)中发现的受低温诱导的b HLH转录因子进行了生物信息学分析,并以层积苹果种子和苹果芽为试材,用半定量和实时定量PCR技术分析其花芽休眠不同阶段的表达变化。蛋白质二级结构预测结果表明,MdCIbHLH1蛋白中以α螺旋和无规则卷曲为主,β折叠和延伸链较少,其中α螺旋145个(27.31%)、β折叠19个(3.58%)、无规则卷曲310个(58.38%)、延伸链57个(10.73%)。半定量和定量结果显示,在层积的苹果种子和休眠的花芽中MdCIbHLH1有相同的表达模式,在休眠解除之前表达较高,随着休眠的解除其表达量下调。因此推测MdCIbHLH1的表达对层积的苹果种子或者花芽休眠及解除具有调控作用。
The bioinformatics analysis of cryogenic-induced b HLH transcription factors found in apple (Malus × domestica Borkh.) Was carried out in the early stage, and the apple seeds and apple buds were used as experimental materials. Semi-quantitative and real-time quantitative PCR The changes of flower bud dormancy at different stages were analyzed. The results of secondary structure prediction showed that the α-helix and random coil were the main components of MdCIbHLH1 protein, with fewer β-sheets and extended chains, including 145 α-helices (27.31%) and 19 β-sheets (3.58% 310 curly (58.38%), 57 extended chain (10.73%). Semiquantitative and quantitative results showed that MdCIbHLH1 had the same expression pattern in stratified apple seeds and dormant flower buds, which was higher before dormancy release and downregulated with dormancy release. Therefore, it is speculated that the expression of MdCIbHLH1 has a regulatory effect on the dormancy and release of the stomached apple seeds or flower buds.