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目的:体外分离培养的胚胎大鼠神经干细胞(NSCs),移植入患有舞蹈症的转基因大鼠的纹状体内的存活、分化情况及对大鼠脑内多巴胺含量的影响。方法:无菌条件下剖腹取5只SD胚胎大鼠(孕15 d)剥离出整个脑组织,进行体外神经干细胞的扩增培养,收集细胞悬液用自动微量注射器垂直刺入大鼠纹状体。结果:胚胎大鼠脑组织中分离的NSCs,可在体外稳定扩增,并且具有分化为神经元的能力,将标记的NSCs细胞悬液移植入大鼠纹状体,移植后5个月,免疫组化检测发现损伤的多巴胺神经元处显示酪氨酸羟化酶呈阳性的神经元。结论:在受损的纹状体移植入NSCs后,NSCs可被诱导分化为多巴胺神经元,进行功能重建。可见应用体外扩增的NSCs移植治疗神经性疾病是可行和有效的。
OBJECTIVE: To investigate the survival and differentiation of neural stem cells (NSCs) of embryonic rat embryos cultured in vitro and their survival and differentiation in the striatum of transgenic rats with chorea and their effects on dopamine content in rat brain. Methods: A total of 5 SD embryos (15 days pregnant) were dissected out of the whole brain tissue under aseptic conditions. The neural stem cells were expanded and cultured in vitro. The cell suspension was collected and penetrated perpendicularly into the striatum of rat by automatic microinjection . Results: NSCs isolated from embryonic rat brain tissue could stably proliferate in vitro and had the ability to differentiate into neurons. The labeled NSCs were transplanted into the striatum of rats. Five months after transplantation, Histochemical tests revealed tyrosine hydroxylase-positive neurons at the lesioned dopamine neurons. CONCLUSIONS: NSCs can be induced to differentiate into dopaminergic neurons for functional reconstruction after transplantation of damaged striatum into NSCs. It can be seen that the application of in vitro expanded NSCs transplantation is feasible and effective in the treatment of neurological diseases.