早在70年代初,Berwald和Sachs报道了用叙利亚金黄地鼠胚胎(SHE)细胞转化试验的结果。随后,许多作者又作了进一步研究。此法敏感性高,但实验条件要求较高,特别是作为饲养层细胞需预先经X射线或~(60)CO照射,而一般的实验室不具备此条件,从而影响了这一方法的推广应用。为此,我们对饲养层细胞的照射条件作了改进,即用紫外线来代替X射线或~(60)CO照 As early as the early 1970s, Berwald and Sachs reported the results of a Syrian hamster embryo (SHE) cell transformation assay. Subsequently, many authors made further research. This method is highly sensitive, but requires high experimental conditions, especially as feeder cells need to be pre-irradiated by X-rays or ~ (60) CO, while the general laboratory does not have this condition, thus affecting the promotion of this method application. To this end, we improved the conditions of feeder cells, that is, instead of using X-ray or ~ (60) CO