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目的观察增生性玻璃体视网膜病变玻璃体切割物的细胞凋亡情况。方法收集６０例不同分级的增生性玻璃体视网膜病变患者的玻璃体切割物，核苷酸末端转移酶介导ｄＵＴＰ缺口翻译法（ＴｄＴ－ｍｅｄｉａｔｅｄｄＵＴＰｎｉｃｋｅｎｄｌａｂｅｌｉｎｇｍｅｔｈｏｄ，ＴＵＮＥＬ法）标记凋亡细胞，光镜观察。结果６０例患者的玻璃体切割物均有细胞凋亡的特征性改变，随着病变程度的加重，非色素细胞凋亡总数逐渐减少，并出现色素细胞凋亡。结论增生性玻璃体视网膜病变存在不同类型细胞的凋亡，细胞凋亡是调控其病变程度的重要机制之一。
Objective To observe the apoptosis of vitreoretinal cells in proliferative vitreoretinopathy. Methods Sixty vitrectomy specimens were collected from patients with proliferative vitreoretinopathy with different grading. Apoptotic cells were labeled with nucleotides transferase-mediated dUTP nick-end labeling (TUNEL) and observed by light microscopy. Results The vitreous incisions of 60 patients all had the characteristic changes of apoptosis. With the severity of the lesion, the total number of non-pigment cells decreased and the apoptosis of pigment cells appeared. Conclusion There are different types of cell apoptosis in proliferative vitreoretinopathy. Apoptosis is one of the important mechanisms that regulate the degree of the disease.