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目的:用气相色谱法测定“冬病夏治”贴膏中的冰片含量。方法:以水杨酸甲酯为内标物,采用内标物预先加入法,弹性石英毛细管柱Agilent HP-5(30m×0.32mm,0.25μm),FID检测器,氮气为载气,柱温为100℃。结果:在该色谱条件下,冰片中异龙脑和龙脑及内标物水杨酸甲酯均得到良好的分离,冰片进样量在0.2785～5.5700mg/mL范围内与峰面积线性关系良好(r=0.9999),冰片的回收率为99.86%(RSD=2.01%)。结论:该法灵敏、准确,重复性好,可用于控制该制剂的质量。
Objective: To determine the content of borneol in “winter sickness summer cure” patch by gas chromatography. Methods: Methyl salicylate was used as an internal standard. The samples were pre-added with an internal standard. The column was equipped with a flexible quartz capillary column Agilent HP-5 (30m × 0.32mm, 0.25μm), a FID detector and nitrogen as the carrier gas. 100 ° C. Results: Under the chromatographic conditions, isoborneol and borneol and methyl salicylate were well separated in borneol. The linear range of the borneol injection was 0.2785 ~ 5.5700 mg / mL (r = 0.9999). The recovery rate of borneol was 99.86% (RSD = 2.01%). Conclusion: The method is sensitive, accurate and reproducible and can be used to control the quality of the preparation.