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提高水稻的氮素利用率对农业生产极为重要,水稻谷氨酸合酶(GOGAT,EC1.4.1.14)被认为具有提高水稻氮素利用率的潜力,但该酶在水稻中的功能以及其对碳氮代谢的影响一直未被系统的报道.本研究以抑制表达谷氨酸合酶基因的转基因水稻为材料,结合谷氨酸合酶基因家族全生育期表达谱数据,研究该基因家族在水稻体内的功能及其对碳氮代谢的影响.结果表明,谷氨酸合酶家族基因成员的表达模式各不相同,具有明显的组织和器官特异性,表明其在体内行使着不同的功能.与野生型相比,抑制表达谷氨酸合酶基因的转基因植株的分蘖数、地上部干重以及单株产量显著下降.同时,转基因植株叶片中的硝酸盐、部分游离氨基酸、叶绿素、糖、糖磷酸以及吡啶核苷酸含量也显著降低,但游离铵、α-酮戊二酸以及异柠檬酸含量上升.分析表明,谷氨酸合酶在水稻的碳氮代谢过程中扮演着重要角色,是水稻氮素高同化过程中必不可少的因子.
Increasing the nitrogen use efficiency of rice is very important for agricultural production. Rice glutamate synthase (GOGAT, EC1.4.1.14) is considered to have the potential to improve nitrogen utilization efficiency in rice, but its function in rice and its On the metabolism of carbon and nitrogen has not been systematically reported.In this study, the inhibition of expression of glutamic acid synthase gene transgenic rice as the material, combined with glutamic acid synthase gene family full-life expression profile data to study the gene family in The function of rice in vivo and its effect on the metabolism of carbon and nitrogen were studied.The results showed that the expression patterns of glutamic acid synthase family members were different from each other and had obvious tissue and organ specificities indicating that they had different functions in vivo. Compared with the wild type, the number of tillers, dry weight above the ground and yield per plant of the transgenic plants expressing glutamate synthase gene were significantly decreased, while nitrate, some free amino acids, chlorophyll, sugar, The contents of free and ammonium, α-ketoglutarate and isocitrate also increased significantly.The results showed that glutamate synthase could be used in the process of carbon and nitrogen metabolism in rice Play an important role in the process of nitrogen assimilation in rice is an essential factor.