蚕丝蛋白经高压高温水脱胶后所得的丝胶溶液 ,经过纯化、浓缩以及喷雾干燥 ,制成丝胶蛋白粉末 .这种丝胶蛋白分子质量高达 2 0 0ku ,其粉末呈白色 ,平均粒度 10 μm ,为热水溶性蛋白 .以这种丝胶蛋白粉末为载体 ,用戊二醛为交联剂 ,制成固定化 L 天冬酰胺酶 .对这种固定化酶活性和动力学性质进行初步研究和分析 ,结果表明这种固定化酶性能稳定 ,对热的稳定性有所提高 ,并具有较好的操作稳定性 ,抗胰蛋白酶水解能力大大提高 The sericin solution obtained by the degumming of silk protein under high pressure and high temperature water is purified, concentrated and spray-dried to prepare a sericin protein powder with a molecular weight of up to 200 ku, the powder is white and the average particle size is 10 μm , As a hot water soluble protein.Using this sericin powder as a carrier and glutaraldehyde as a crosslinking agent, an immobilized L asparaginase was prepared. The activity and kinetic properties of the immobilized enzyme were studied The results showed that this immobilized enzyme has stable properties, increased stability to heat, better operation stability and greatly improved antitrypsin hydrolysis ability