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目的探讨血管内皮生长因子(VEGF)基因多态性与支气管肺发育不良(BPD)相关性。方法选自2009-07-01—2012-02-01华中科技大学同济医学院附属同济医院收治的符合BPD诊断标准的患儿64例(BPD组)和同期住院的无肺部疾病患儿106例(对照组)。利用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法分析两组VEGF+405、-2578基因频率分布,酶联免疫吸附试验(ELISA)分析BPD组血浆VEGF浓度。结果 (1)VEGF+405G>C:BPD组中G的基因型频率和等位基因频率均高于对照组,差异有统计学意义(P<0.05);VEGF-2578C>A:BPD组中A的基因型频率和等位基因频率均高于对照组,差异有统计学意义(P<0.05)。(2)BPD组VEGF+405GG/GC血浆表达低于VEGF+405CC(P<0.05),VEGF-2578CC/CA血浆表达与VEGF-2578AA差异无统计学意义(P>0.05)。结论 VEGF+405、-2578基因多态性和BPD相关,推测其和BPD遗传易感性相关。VEGF+405多态性与VEGF血浆表达相关,血浆中VEGF减低可能是BPD发病机因素之一。
Objective To investigate the relationship between vascular endothelial growth factor (VEGF) gene polymorphism and bronchopulmonary dysplasia (BPD). Method selected from 2009-07-01-2012-02-01 Tongji Medical College Affiliated Tongji Hospital Huazhong University of Science and Technology in line with BPD diagnostic criteria 64 cases of children (BPD group) and hospitalized in the same period without pulmonary disease in 106 cases (Control group). The frequencies of VEGF + 405 and -2578 genes in two groups were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and the plasma concentrations of VEGF in BPD patients were analyzed by enzyme linked immunosorbent assay (ELISA). Results (1) The frequencies of genotypes and alleles of G in VEGF + 405G> C: BPD group were significantly higher than those in control group (P <0.05), while VEGF-2578C> A: The genotype frequency and allele frequency were higher than the control group, the difference was statistically significant (P <0.05). (2) The plasma level of VEGF + 405GG / GC in BPD group was lower than that in VEGF + 405CC group (P <0.05). There was no significant difference between VEGF-2578CC / CA plasma level and VEGF-2578AA group (P> 0.05). Conclusion The polymorphisms of VEGF + 405 and -2578 correlate with BPD, suggesting that they are associated with genetic susceptibility to BPD. VEGF + 405 polymorphism is associated with the expression of VEGF in plasma, and the decrease of VEGF in plasma may be one of the pathogenesis of BPD.