【摘 要】
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Objective:To examine the individual and synergistic anti-listerial effect of nisin and leaf essential oil ofMetasequoia glyptostroboides(M. glyptostroboides) ag
【机 构】
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Department of Applied Microbiology and Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-
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Objective:To examine the individual and synergistic anti-listerial effect of nisin and leaf essential oil ofMetasequoia glyptostroboides(M. glyptostroboides) against one of the leading foodbe pathogensListeria monocytogenes(L. monocytogenes)ATCC19116 in milk samples. Methods:The whole(8%), low(1%) and skim(no fat content) milk samples were inoculated withL. monocytogenesATCC19116 along with leaf essential oil ofM. glyptostroboides or nisin alone as well in combinations.Results:In this study, the leaf essential oil at the concentrations of2% and5% revealed strong anti-listerial effect againstL. monocytogenesATCC19116 in all categories of milk samples.Nisin at the concentrations of250 and500IU/mL displayed a strong inhibitory effect againstATCC19116 as compared to the control group.Additionally, synergistic combinations of leaf essential oil(1%) and nisin(62.5,125,250 and500IU/mL) also had a remarkable anti-listerial synergism in all the tested milk samples including whole, low and skim milk after14 days.Conclusions:As a major finding, the leaf essential oil ofM. glyptostroboides might be a useful candidate for using in food industry to control the growth of foodbe pathogenic bacteria as confirmed by its potent anti-listerial synergistic effect with nisin againstL. monocytogenesATCC19116 in different milk samples.
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