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[目的]探索一种采用球囊损伤联合高脂饮食的方法建立大鼠腹主动脉内膜损伤,诱导大鼠腹主动脉粥样硬化动物模型,旨在为研究者们提供一种更能贴切模拟动脉粥样硬病变的动物模型,为AS发病机制探讨和治疗方法的探索提供与临床病例更为相似的实验对象。[方法]SD大鼠20只,随机分为空白对照组和模型组,每组10只,空白对照组正常饲养,不做任何处理。模型组以高脂饲料喂养4周,自由饮水。4周后,行腹主动脉球囊损伤术,术后继续高脂饲料喂养6周。实验结束后对两组大鼠腹主动脉进行病理切片,观察其病理变化。[结果]光镜下观察,模型组大鼠腹主动脉可见粥样斑块病理改变。[结论]高脂联合动脉内膜机械性损伤方法复制动脉粥样硬化大鼠模型具有方法可靠,可重复性高,更符合临床动脉粥样硬化疾病的发生发展过程等优点。
[Objective] To explore an animal model of abdominal aortic atherosclerosis induced by intima injury of the abdominal aorta and balloon injury combined with high-fat diet in rats. The purpose is to provide a more appropriate Animal models simulating atherosclerosis provide an experimental target for exploring the pathogenesis of AS and exploring the treatment methods more closely related to clinical cases. [Methods] Twenty SD rats were randomly divided into blank control group and model group with 10 rats in each group. The blank control group was maintained normally without any treatment. The model group was fed with high-fat diet for 4 weeks, drinking water freely. After 4 weeks, abdominal aortic balloon injury was performed and the high-fat diet was continued for 6 weeks after operation. At the end of the experiment, the abdominal aorta of rats in both groups were pathologically sectioned and their pathological changes were observed. [Result] Under light microscope, pathological changes of atherosclerotic plaque were observed in abdominal aorta of model rats. [Conclusion] The method of high-fat combined with arterial intimal mechanical injury in rat model of atherosclerosis has the advantages of reliable method, high repeatability and more in line with the occurrence and development of clinical atherosclerotic disease.