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目的探讨银杏内酯A在大鼠急性脊髓损伤后对神经纤维修复和运动功能恢复的作用。方法 28只SD大鼠随机分为高剂量银杏内酯A组(A1组,6只)、中剂量银杏内酯A组(A2组,6只)、低剂量银杏内酯A组(A3组,6只)、损伤模型组(B组,5只)和假手术组(C组,5只)。损伤后不同时间段对大鼠进行BBB行为学评分,损伤后30d取脊髓损伤区域进行免疫荧光组织化学染色和电镜检测。结果 A1、A2、A3组损伤后的BBB评分均高于B组(P<0.05),A1、A2组BBB评分高于A3组(P<0.05)。A1、A2、A3组均可见神经微丝蛋白免疫反应阳性的再生神经纤维,B组未见阳性神经纤维。电镜观察显示,A1、A2、A3组损伤区域内均可见神经纤维,且A1、A2组的有髓神经纤维数目多于A3组(P<0.05)。结论银杏内酯A在大鼠急性脊髓损伤后能促进神经纤维的修复和运动功能的恢复,且高、中剂量银杏内酯A治疗效果优于低剂量银杏内酯A。
Objective To investigate the effect of ginkgolide A on recovery of nerve fibers and recovery of motor function after acute spinal cord injury in rats. Methods Twenty-eight Sprague-Dawley rats were randomly divided into high dose ginkgolide A group (group A1, n = 6), middle dose ginkgolide A group (group A2, n = 6), low dose ginkgolide A group 6), injury model group (B group, 5 rats) and sham operation group (C group, 5 rats). The BBB behavioral score of rats was evaluated at different time points after injury, and the area of spinal cord injury was taken 30 days after injury for immunocytochemical staining and electron microscopy. Results The scores of BBB in groups A1, A2 and A3 were higher than those in group B (P <0.05). The scores of BBB in groups A1 and A2 were higher than those in group A3 (P <0.05). In group A1, A2, and A3, nerve fibers positive for neurofilament immunoreactivity were seen, while in group B, no positive nerve fibers were found. Electron microscopy showed that nerve fibers were found in the injured area of A1, A2 and A3 groups, and the number of myelinated nerve fibers in A1 and A2 groups was more than that in A3 group (P <0.05). Conclusion Ginkgolide A can promote the recovery of nerve fibers and motor function after acute spinal cord injury in rats, and the effect of high and medium dose ginkgolide A is better than that of low dose ginkgolide A.