目前，生产转基因动物主要的方法仍是受精卵显微注射法，所需设备复杂，技术难度大，成功率低。为此，人们都在寻找一种新的方法。利用精子携带外源DNA进入卵母细胞的精子载体法是设想之一。九十年代初，Mare和Rottmann等分别报导了电击法和脂质体转染法让精子在体外携带上外源 At present, the main method of producing transgenic animals is still microinjection of fertilized eggs. The required equipment is complex, the technique is difficult, and the success rate is low. To this end, people are looking for a new way. It is one of the ideas to use the sperm vector method that sperm carry foreign DNA into oocytes. In the early 1990’s, Mare and Rottmann et al. Reported on the electroporation and lipofection methods respectively to carry sperm in vitro