【摘 要】
：
Objective: To investigate the possible role of GSTπ in esophageal carcinogenesis. Methods: GST-πexpression at mRNA level was studied by in situ hybridization
【机 构】
：
Department of Pathology,Henan Key Laboratory of Tumor Pathology
【基金项目】
：
the Key Project of Henan Province Science Foundation
论文部分内容阅读
Objective: To investigate the possible role of GSTπ in esophageal carcinogenesis. Methods: GST-πexpression at mRNA level was studied by in situ hybridization (ISH) and at protein level by immunohistochemistry (IHC). GST-π expression in normal epithelial cells (NC) of the esophagus,hyperplastic cells (HC), dysplastic cells (DC) from grade Ⅰ to Ⅲ, carcinoma in situ (CIS) and all the cells in squamous cell carcinomas (SCC) were examined in the same esophageal cancer specimens (n=48) which provided a model reflecting the process of esophageal carcinogenesis. Results: The positive rate of IHC staining was 87. 5% for NC, 95.3% for HC, 55.9% for DC (grade Ⅰ: 73.9%, grade Ⅱ: 47.4%, grade Ⅲ: 41.2%),36.4% for CIS and 45.8% for SCC. The positive rate of GST-π mRNA expression was 81.2% for NC, 94.4% for HC, 61.9% for DC (grade Ⅰ: 76.5%, grade Ⅱ: 61.5%,grade Ⅲ: 41.7%), 44.4% for CIS and 83.3% for grade ⅠSCC, 30.0% for grade Ⅱ SCC and 0% for grade ⅢSCC. There was no statistically significant difference in GST-π expression at the mRNA and the protein level.Conclusion: There is a decreasing tendency of GST-πexpression from dysplasia to CIS and SCC. The decrease in GST-π expression is an early event in esophageal carcinogenesis.
其他文献
目的 :了解娱乐性ATS使用者的人口学特征 ,使用方式及其他有关问题。方法 :采用病例发现法和易接近对象晤谈法 ,并结合滚雪球的方法 ,以问卷方式收集娱乐性ATS使用者的有关资
In previous studies, we found that EDRF2 was an erythroid differentiation related factor, whose expression was markedly upregulated during erythroid differentia
A charge pump design is presented to operate at 10 kHz with 100 μA in a liquid crystal display (LCD) driver for cell phone. Optimal channel widths are designed
目的:评估纳曲酮院外治疗酒依赖的临床疗效.方法:采用随机对照的方法,分别对纳曲酮组和安慰剂组各23例和22例,作总疗程12周的临床观察.结果:纳曲酮显著降低病人对酒精的渴求
To study the regulation of angiotensin Ⅱ(Ang Ⅱ) on Gαq/11 protein of vascular smooth muscle cell (VSMC) and its underlying mechanism, the protein synthesis w
The theoretical model construction of mRNA hairpin structure and single-stranded structure as well as the simulation studies on RNA structure determined by the
总结了开辟栏目的实践经验,栏目的开办,激发了学生的科研热情,促进了学生对课堂所学理论知识的理解,培养了学生创造思维、动手、写作、外语等多方面的能力,调动了广大学生对
编辑在中医药学术论文审稿过程中经常遇到医案、医话、经验总结、医论等特殊体裁的论文。这些体裁的论文如属临床内容的,蕴含着经验个性化,其质量按国际通用的随机、双盲、对
目的:了解酒依赖者认知功能状况.方法:对完成脱酒治疗的37例男性酒依赖者和34例男性非依赖者进行Wisconsin卡片分类测验(WCST)评定.结果:两组间分类数(t=5.44)、正确应答百分
The structure-based sequence motif of the distant proteins in evolution, protein tyrosine phosphatases (PTP) Ⅰ and Ⅱ superfamilies, as an example, has been de