论文部分内容阅读
目的:观察商陆皂苷甲对水负荷大鼠肾脏水通道蛋白2(AQP2)及水通道蛋白4(AQP4)表达的调节作用,研究商陆皂苷甲利尿作用机制。方法:将50只雄性SD大鼠随机分为空白对照组、氢氯噻嗪组及商陆皂苷甲高、中、低剂量组,腹腔注射给药,氢氯噻嗪组给予氢氯噻嗪0.4 mg/kg,商陆皂苷甲高、中、低剂量组分别给5.2、2.6、1.3 mg/kg商陆皂苷甲,收集并测量给药后6 h内的尿量;水负荷实验结束后将大鼠麻醉处死,运用免疫组化及Real Time-PCR技术检测大鼠肾脏AQP2、AQP4蛋白及mRNA表达的变化。结果:与空白对照组比较,商陆皂苷甲高剂量组大鼠尿量显著增多,肾脏AQP2、AQP4蛋白及mRNA的表达显著下调(P<0.05);商陆皂苷甲中、低剂量组尿量及肾脏AQP2、AQP4蛋白及mRNA表达差异无统计学意义。结论:商陆皂苷甲通过下调大鼠肾脏AQP2、AQP4蛋白及mRNA表达,使水分重吸收降低,可能是其利尿作用的机制之一。
AIM: To observe the regulation of aquaporin 2 (AQP2) and aquaporin 4 (AQP4) expression in the kidney of water-loaded rats by esculentoside A and to study the mechanism of esculentoside meconium-induced diarrhea. Methods: 50 male Sprague-Dawley rats were randomly divided into blank control group, hydrochlorothiazide group and esculentoside A high, medium and low dose group, intraperitoneal injection, hydrochlorothiazide group given hydrochlorothiazide 0.4 mg / kg, , Middle and low dose groups were respectively 5.2,2.6,1.3 mg / kg Esculentoside A collection and measurement of urine output within 6 h after administration; water stress after the experiment the rats were anesthetized and killed by immunohistochemistry and The changes of AQP2 and AQP4 protein and mRNA expression in rat kidney were detected by Real Time-PCR. Results: Compared with the blank control group, the urinary output of AQP2, AQP4 and mRNA were significantly decreased (P <0.05), and the urinary output And kidney AQP2, AQP4 protein and mRNA expression differences were not statistically significant. Conclusion: Esculentoside A may be one of the mechanisms of its diuretic effect by down-regulating the protein and mRNA expression of AQP2 and AQP4 in kidney of rats.