通过盆栽试验,探讨了在田间持水量75%水平下,小麦(Triticum aestivum)根系分泌物丁香酚对小麦、蚕豆(Vicia sativa)单作和间作群体土壤微生物数量及其多样性的影响,以期为化感物质间作群体的调控提供理论依据。结果表明,总体上,微生物的多样性降低,真菌和放线菌数量减少,而细菌数量增加。与无丁香酚处理对比,在3种种植模式中,丁香酚对土壤真菌和放线菌数量均表现为化感抑制作用,两种微生物数量的减小幅度分别为21.95%～98.42%和13.33%～92.82%;单作小麦较间作处理的细菌数量变化了-118.63%～90.73%,但单作蚕豆细菌数量变化了-56.39%～76.76%;放线菌占微生物的相对数量变化了-4.42%～4.32%,细菌变化了-4.36%～4.88%。小麦间作蚕豆降低了土壤微生物多样性指数,较单作小麦、单作蚕豆分别变化了15%～26.98%和-45.45%～6.67%;经丁香酚处理,单作中土壤微生物多样性指数降低,而间作变化不大。 Pot experiments were conducted to investigate the effects of eugenol, a plant root exudates of wheat (Triticum aestivum), on the number and diversity of soil microbes in single and intercropping populations of wheat and Vicia sativa at field level of 75% Allelochemicals intercropping group regulation provide the theoretical basis. The results showed that, overall, the diversity of microorganisms decreased, the number of fungi and actinomycetes decreased, and the number of bacteria increased. Compared with no eugenol treatment, eugenol showed allelopathic effects on soil fungi and actinomycetes in all three planting modes, and the reduction of the two microorganisms was 21.95% -98.42% and 13.33% respectively, ~ 92.82%. The number of bacteria in single-cropping wheat varied from -118.63% to 90.73%, but the number of bacteria in single-cropped broad bean changed by -56.39% -76.76%. The relative amount of actinomycetes in soil changed by -4.42% ~ 4.32%, bacteria changed -4.36% ~ 4.88%. Wheat intercropping broad bean reduced soil microbial diversity index, which was 15% ~ 26.98% and -45.45% ~ 6.67%, respectively, compared with that of monoculture wheat and single monocotyledon. After being treated with eugenol, the index of soil microbial diversity decreased, The intercropping little change.