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本文以自然授粉的‘春蕾’、‘沪017’、‘布目早生’、‘1-4-8’、‘7-6-9’的胚珠为试材,研究了早熟和特早熟桃胚珠培养胚成苗的有关影响因素。本试验5个品种(品系)的结果表明,桃品种或品系的果实生育期愈短,胚珠培养胚的成苗率愈高,特早熟桃‘春蕾’花后48天的胚珠培养在改良S H培养基附加BA1.0mg/1+IAA1.0mg/1上30天,将长大的幼胚转移至TM培养基上,胚的成苗率达75％。此外,作者对胚珠培养技术在桃育种中的应用范围及其培养程序开展了讨论。
In this study, ovules of ’Chunlei’, ’Shanghai 017’, ’Scutum’, ’1-4-8’, and ’7-6-9’ pollinated by natural pollination were used as experimental materials to study the effects of precocious and extra-peach ovule culture Related Factors of Embryogenesis. The results of five varieties (lines) of this experiment showed that the shorter the growth period of the peach cultivars or lines, the higher the seedling rate of ovule culture embryos, the more precocious peach ’Chunlei’ The culture medium was supplemented with BA1.0mg / 1 + IAA1.0mg / 1 for 30 days. The grown immature embryos were transferred to TM medium, and the embryo germination rate reached 75%. In addition, the authors discussed the application of ovule culture techniques in peach breeding and its cultivation procedure.