论文部分内容阅读
为探索UV-B辐射胁迫对大豆幼苗的伤害机制,采用水培法研究紫外辐射(UV-B,280～320 nm)胁迫作用对大豆(Glycine max)幼苗叶片气孔密度、气孔导度(G_S)、脱落酸(ABA)含量、过氧化氢(H_2O_2)含量和过氧化氢酶(CAT)活性的影响。静态实验结果表明,UV-B辐射处理降低了大豆幼苗气孔密度,UV-B处理组气孔密度低于对照CK(喷水)组。动态曲线显示,较之CK(喷水),UV-B辐射使大豆幼苗G_S下降,且最终达到较好的恢复效果;ABA和H_2O_2含量、CAT活性的变化趋势与G_S的变化趋势不同,与CK(喷水)相比,UV-B辐射导致ABA和H_2O_2积累,CAT活性提高。研究表明,UV-B辐射抑制大豆幼苗水分代谢。
In order to explore the mechanism of injury to soybean seedling by UV-B radiation, the effects of UV-B radiation (280-320 nm) on stomatal density, stomatal conductance (G-S) , ABA content, H 2 O 2 content and catalase (CAT) activity were studied. The results of static experiments showed that the stomatal density of soybean seedling decreased with UV-B radiation treatment, and the stomatal density of UV-B treatment group was lower than CK group. The dynamic curves showed that compared with CK (sprayed water), the UV-B radiation reduced the G_S of soybean seedlings and finally achieved a better recovery effect. The change trend of ABA and H_2O_2 content and CAT activity was different from that of G_S, (Water spray), UV-B radiation led to the accumulation of ABA and H_2O_2, CAT activity increased. Studies have shown that UV-B radiation inhibition of soybean seedling water metabolism.