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目的探讨烧伤对小鼠棕色脂肪组织(brown adipose tissue,BAT)的影响。方法取3~4月龄雄性BALB/c小鼠40只,体重(20±3)g,随机分为实验组和对照组(n=20)。实验组小鼠将背部置于90℃热水9 s制备30%总体表面积Ⅲ度烫伤模型,对照组小鼠背部置于37℃热水中9 s,作为对照。造模前后检测小鼠空腹体重、体温;造模后7 d取BAT及白色脂肪组织(white adipose tissue,WAT)称重并行大体、组织学及透射电镜观察,实时定量PCR及免疫组织化学染色检测脂肪组织解偶联蛋白1(uncoupling protein 1,UCP-1)基因及蛋白表达水平。结果造模前两组小鼠空腹体重、体温比较,差异均无统计学意义(P>0.05);造模后1、2、3、4周实验组小鼠空腹体重显著低于对照组(P<0.05),1、2、3、7 d实验组体温显著高于对照组(P<0.05)。造模后7 d,与对照组相比,实验组WAT重量明显降低、BAT重量增加(P<0.05),WAT及BAT细胞均变小,细胞数增多,细胞质及线粒体较紧密;实验组BAT及WAT中UCP-1基因相对表达量及蛋白表达均显著高于对照组(P<0.05)。结论小鼠烧伤后BAT参与了机体的高代谢反应。
Objective To investigate the effects of burn on brown adipose tissue (BAT) in mice. Methods Forty male BALB / c mice (3-4 months old) weighing 20 ± 3 g were randomly divided into experimental group and control group (n = 20). The mice in the experimental group were placed in the hot water at 90 ° C for 9 s to prepare a 30% total surface area Ⅲ degree scald model. The control mice were placed in hot water at 37 ° C for 9 s as the control. Fasting body weight and body temperature of mice were measured before and after modeling. BAT and white adipose tissue (WAT) were weighed at 7 days after operation. Gross, histological and transmission electron microscopic observations, real-time quantitative PCR and immunohistochemical staining The uncoupling protein 1 (UCP-1) gene and protein expression levels in adipose tissue were detected. Results There was no significant difference in fasting body weight and body temperature between the two groups before modeling (P> 0.05). After 1, 2, 3 and 4 weeks, the fasting body weight of mice in the experimental group was significantly lower than that of the control group (P <0.05). The body temperature of experimental group was significantly higher than that of control group on 1,2,3,7 d (P <0.05). Compared with the control group, the weight of WAT and the weight of BAT in experimental group were significantly decreased (P <0.05), the number of WAT and BAT cells were smaller, the number of cells increased and the cytoplasm and mitochondria were more dense in the experimental group than those in the control group The relative expression and protein expression of UCP-1 in WAT were significantly higher than those in control group (P <0.05). Conclusion BAT in mice is involved in the high metabolic reaction in mice.