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目的:研究光枝勾儿茶全株中的红镰霉素苷类成分,探讨其对二苯代苦味基肼自由基(DPPH)的清除作用。方法:采用硅胶柱色谱,Sephadex LH-20凝胶柱色谱进行分离纯化,通过波谱学方法鉴定化合物结构,并对化合物进行清除DPPH活性试验。结果:从光枝勾儿茶乙醇提取物的正丁醇部分分离得到3个红镰霉素苷类化合物,分别鉴定为红镰霉素-6-O-β-D-吡喃葡萄糖苷(rubrofusarin-6-O-β-D-glucopyranoside,1),红镰霉素-6-O-β-D-(6’-O-乙酰基)吡喃葡萄糖苷[rubrofusarin-6-O-β-D-(6’-O-acetyl)glucopyranoside,2],红镰霉素-6-O-α-L-鼠李糖基-(1-6)-O-β-D-吡喃葡萄糖苷[rubrofusarin-6-O-α-L-rhamnosyl-(1-6)-O-β-D-glucopyranside,3]。对DPPH清除能力试验结果表明:3个化合物对DPPH自由基都有较强的清除能力,对照品VitC、化合物1～3的半清除率浓度IC50(μmol.L-1)大小依次为18.2,40.5,23.3,13.6。结论:化合物1～3均为首次从该植物中分离得到,其中化合物2为新化合物。3个化合物均具有较强的抗氧化活性,其中化合物3的清除效果优于VitC。
OBJECTIVE: To study the composition of erythromycin glycosides in the whole plant of Camellia oleifera, and to explore its scavenging effect on the diphenyl substituted bitter base hydrazine free radical (DPPH). Methods: The compounds were separated and purified by silica gel column chromatography and Sephadex LH-20 column chromatography. The structures of compounds were identified by spectroscopic methods and the compounds were scavenged for DPPH activity. Results: Three erythromycin glycosides were isolated from the n-butanol fraction of Ethanol extract of C. lucidum and identified as rubrofusarin-6-O-β-D-glucopyranoside 6-O-β-D-glucopyranoside, 1) rubrofusarin-6-O-β-D- (6’-O-acetyl) glucopyranoside, 2], rubidazole-6-O-α-L-rhamnosyl- 6-O-α-L-rhamnosyl- (1-6) -O-β-D-glucopyranside, 3]. The DPPH scavenging ability test results showed that all three compounds had strong scavenging ability on DPPH free radicals. The concentration of IC50 (μmol.L-1) of the reference substance VitC and compounds 1 ~ 3 was 18.2,40.5 , 23.3,13.6. Conclusion: Compounds 1 ~ 3 were isolated from this plant for the first time, of which compound 2 is a new compound. Three compounds have strong antioxidant activity, of which compound 3 scavenging effect is better than VitC.