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目的:通过比较慢性宫颈炎,CINⅠ,CINⅡ,CINⅢ和宫颈鳞癌中NF-κB与VEGF的表达情况,探讨NF-κB与VEGF在宫颈癌发生发展中的作用及他们相互之间的关系。方法:采用免疫组织化学技术(SP法)检测NF-κB以及VEGF在15例慢性宫颈炎,42例宫颈上皮内瘤样病变(CIN)和51例宫颈鳞癌(SCCs)患者中的表达。结果:免疫组织化学显示,在炎症组,CINⅠ～Ⅱ,CINⅢ和宫颈鳞癌中NF-κB与VEGF的组织学评分呈逐级递增关系。在宫颈鳞癌中NF-κB、VEGF表达强度与病理分型无关,而与病理分级及淋巴结转移与否有关,且随病理分级的增高NF-κB与VEGF的组织学评分呈逐级递增关系。而淋巴结转移(+)组与淋巴结转移(-)组之间比较有显著性差异(P<0.01)。结论:NF-κB的异常激活是促进从慢性宫颈炎到宫颈癌这一过程向前发展的重要因素,NF-κB还可能通过上调VEGF表达而促进肿瘤的血管生成来促进癌症发生及淋巴结转移。
OBJECTIVE: To investigate the role of NF-κB and VEGF in the development and progression of cervical cancer and their relationship with each other by comparing the expression of NF-κB and VEGF in chronic cervicitis, CINⅠ, CINⅡ, CINⅢ and cervical squamous cell carcinoma. Methods: The expressions of NF-κB and VEGF in 15 cases of chronic cervicitis, 42 cases of cervical intraepithelial neoplasia (CIN) and 51 cases of cervical squamous cell carcinoma (SCC) were detected by immunohistochemistry (SP method). Results: Immunohistochemistry showed that the histological score of NF-κB and VEGF in the inflammatory group, CINⅠ-Ⅱ, CINⅢ and cervical squamous cell carcinoma were gradually increased. The expression of NF-κB and VEGF in cervical squamous cell carcinoma was not related to the pathological classification, but correlated with pathological grade and lymph node metastasis. The histological score of NF-κB and VEGF increased progressively with the pathological grade. There was significant difference between lymph node metastasis (+) group and lymph node metastasis (-) group (P <0.01). Conclusion: Abnormal activation of NF-κB is an important factor to promote the progression from chronic cervicitis to cervical cancer. NF-κB may promote the carcinogenesis and lymph node metastasis by up-regulating VEGF expression and promoting tumor angiogenesis.