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目的探讨α亚族趋化因子受体3(CXC-chemokine receptor 3,CXCR3)在不同人舌鳞癌细胞株的表达以及其配体干扰素诱导蛋白-10(interferon induced protein 10,IP-10)对人舌鳞癌细胞株CAL-27增殖和凋亡的影响。方法采用蛋白印迹法和免疫荧光染色对3种人舌鳞癌细胞株(CAL-27、UM-1、Tca-8113)IP-10的相应受体CXCR3的表达进行检测。CAL-27细胞被分为3个实验组和1个对照组,3个实验组根据IP-10的刺激浓度,分为10 ng/mL组、20 ng/mL组、40 ng/mL组,对照组不予刺激。12 h、24 h、48 h时检测4组细胞的增殖;24 h时用流式细胞仪检测细胞的凋亡。结果 CXCR3在3种人舌鳞癌细胞株均有表达,并且CXCR3在3株细胞的胞膜及胞质内均有染色。和对照组相比,12 h、24 h时,3种浓度的IP-10均能够促进CAL-27细胞增殖(P<0.05);在48 h时,只有40 ng/mL的IP-10能够促进CAL-27细胞增殖(P<0.05),10 ng/mL、20 ng/mL的IP-10对CAL-27细胞的增殖无促进作用(P>0.05)。在24 h时,对照组、10 ng/mL组、20 ng/mL组和40 ng/mL组的细胞凋亡率分别为(0.053 3±0.472 6)%、(3.236 7±0.940 0)%、(4.516 7±1.115 4)%和(3.363 3±0.571 2)%,3种浓度IP-10均能够促进CAL-27细胞的凋亡(P<0.05),但3个实验组之间差异无统计学意义(P>0.05)。结论CXCR3在3种人舌鳞癌细胞株的胞膜及胞质内均有表达;IP-10既能促进CAL-27细胞的增殖,又能促进其凋亡。随着时间的延长,IP-10的促增殖作用减弱,而这种减弱可能是由IP-10的促凋亡作用的逐步发挥所引起的。
Objective To investigate the expression of CXC-chemokine receptor 3 (CXCR3) in different human tongue squamous cell carcinoma cell lines and its interferon-induced protein 10 (IP-10) Effects on proliferation and apoptosis of human tongue squamous cell carcinoma cell line CAL-27. Methods Western blotting and immunofluorescence staining were used to detect the expression of CXCR3 in three human tongue squamous cell carcinoma cell lines (CAL-27, UM-1, Tca-8113) IP-10. CAL-27 cells were divided into three experimental groups and one control group. The three experimental groups were divided into 10 ng / mL group, 20 ng / mL group and 40 ng / mL group according to IP-10 stimulation concentration. Group does not stimulate. The proliferation of 4 groups of cells was detected at 12 h, 24 h and 48 h, and the apoptosis was detected by flow cytometry at 24 h. Results CXCR3 was expressed in all three human tongue squamous cell carcinoma cell lines, and CXCR3 was stained in both cell membrane and cytoplasm. Compared with the control group, IP-10 at three concentrations all promoted the proliferation of CAL-27 cells at 12 h and 24 h (P <0.05). Only IP-10 at 40 ng / mL at 48 h promoted CAL-27 cell proliferation (P <0.05). The proliferation of CAL-27 cells was not enhanced by 10 ng / mL and 20 ng / mL IP-10. At 24 h, the apoptotic rates in control, 10 ng / mL, 20 ng / mL and 40 ng / mL groups were (0.053 3 ± 0.472 6)%, (3.236 7 ± 0.940 0)%, (4.516 7 ± 1.115 4)% and (3.363 3 ± 0.571 2)%, respectively. All the three concentrations of IP-10 could promote the apoptosis of CAL-27 cells (P <0.05) Significance (P> 0.05). Conclusions CXCR3 is expressed both in the cytoplasm and cytoplasm of three human tongue squamous cell carcinoma cell lines. IP-10 can promote the proliferation of CAL-27 cells and promote its apoptosis. With the prolongation of IP-10, the pro-proliferative effect weakened, and this decrease may be caused by the progressive activation of IP-10 pro-apoptotic effect.