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[目的]为mtDNA-RAPD技术检测杂种后代的纯度的可行性提供参考,为引物组合法广泛应用于植物纯度检测、遗传多样性分析提供依据。[方法]应用6对随机单引物和引物组合对99B和海岛棉及其F2代的mt DNA进行RAPD扩增并进行多态性分析。[结果]应用单引物对F2代mtDNA进行RAPD检测多态性分析,从6个单引物RAPD-PCR扩增得到了3个差异株,结果表明该群体的纯度为95%,应用引物组合扩增多态性分析表明,从15对引物RAPD-PCR扩增检测到4个差异株,该群体的纯度为93%。表明引物组合扩增多态性分析具有准确性高、精确性好的优点,也表明在植物种质纯度检测中引物组合法的优势及利用价值。[结论]mtDNA的多态性检测具有很好应用价值和应用前景。
[Objective] The aim of the study was to provide reference for the feasibility of using mtDNA-RAPD to detect the purity of hybrid progeny and to provide a basis for primer combination method widely used in plant purity test and genetic diversity analysis. [Method] Six pairs of random single primer and primer combinations were used to amplify mt DNA of 99B and G. barbadense and their F2 generation and to analyze the polymorphism. [Result] Using single primer to analyze the F2 generation mtDNA RAPD polymorphism, three different strains were amplified from 6 single primers by RAPD-PCR. The results showed that the purity of this group was 95% Polymorphism analysis showed that 4 different strains were detected by RAPD-PCR amplification from 15 pairs of primers, and the purity of this population was 93%. The results showed that the primer combination amplification polymorphism analysis has the advantages of high accuracy and good accuracy, and also shows the advantages and utilization value of the primer combination method in the test of plant germplasm purity. [Conclusion] The detection of mtDNA polymorphism has good application value and application prospect.