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为研究氧化型低密度脂蛋白和维生素Ｅ对人脐静脉内皮细胞产生一氧化氮及合酶的影响，应用一氧化氮及一氧化氮合酶试剂盒测定培养的人脐静脉内皮细胞一氧化氮含量及一氧化氮合酶活性。结果发现，氧化型低密度脂蛋白能显著抑制培养的内皮细胞一氧化氮的产生，从对照组的１２６±２４ｍｍｏｌ／ｇ下降为４１±８ｍｍｏｌ／ｇ；并能明显降低一氧化氮合酶的活性，具有浓度和时间效应。抗氧化剂维生素Ｅ显著增加氧化型低密度脂蛋白刺激内皮细胞产生一氧化氮含量以及一氧化氮合酶活性。提示氧化型低密度脂蛋白可以通过抑制内皮细胞一氧化氮合酶的活性而抑制其产生一氧化氮。维生素Ｅ通过增加一氧化氮合酶活性而增加内皮细胞产生一氧化氮。
In order to study the effects of oxidized low density lipoprotein and vitamin E on nitric oxide and synthase production in human umbilical vein endothelial cells, nitric oxide and nitric oxide synthase kits were used to measure nitric oxide Content and nitric oxide synthase activity. The results showed that oxidized low-density lipoprotein could significantly inhibit the production of nitric oxide in cultured endothelial cells from 126 ± 24mmol / g in the control group to 41 ± 8mmol / g; and significantly reduced the activity of nitric oxide synthase , With concentration and time effect. Antioxidant vitamin E significantly increased oxidized low density lipoprotein to stimulate endothelial nitric oxide production and nitric oxide synthase activity. Prompted oxidized low-density lipoprotein can inhibit nitric oxide production by inhibiting the activity of endothelial nitric oxide synthase. Vitamin E increases endothelial nitric oxide production by increasing nitric oxide synthase activity.