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[目的]研究硝基多环芳香烃(1-Nitropyrene,1-NP)对大鼠呼吸道上皮的致损性细胞形态学变化,为人类呼吸系统、免疫系统等疾病的流行病学病因提供依据。[方法]以气管灌入法将不同化学浓度的1-NP注射入大鼠的气管,每周一次,连续15周后,以透射电子显微镜来观察气管上皮细胞的超微结构变化。[结果]大鼠的呼吸道上皮出现化生、细胞凋亡,同时伴有基底膜不连续、不完整,有更加恶化的现象,并出现剂量依赖反应。[结论]呼吸道是吸入致癌物的主要标的物,而1-NP对大鼠上皮细胞的影响值得关注。
[Objective] To investigate the morphological changes of rat respiratory epithelial cells induced by 1-Nitropyrene (1-NP) and provide the basis for the epidemiological etiology of human respiratory system and immune system. [Methods] 1-NP with different chemical concentrations was injected into the trachea of rats by tracheal irrigation once a week for 15 weeks. The ultrastructural changes of tracheal epithelial cells were observed by transmission electron microscopy. [Results] Rat respiratory epithelial cells appeared metaplasia, apoptosis, accompanied by the basement membrane is not continuous, incomplete, there is a worsening phenomenon, and a dose-dependent response. [Conclusion] The respiratory tract is the main target substance for inhalation of carcinogens, and the effect of 1-NP on rat epithelial cells deserves attention.