论文部分内容阅读
目的调查分析中国28个省/区汉族人群41个STR基因座的遗传多态性,为相关研究和鉴定提供全面科学的基础数据。方法收集28个省/区汉族9 126名无关个体血样本,采用Global FilerTM、AGCU EX-22和AGCU 21+13种试剂盒,进行41个STR基因座分型,统计各基因座等位基因分布和遗传学参数,并对各数据之间进行差异性检验。结果中国28个省/区汉族人群41个基因座分别检出8~76个等位基因,各基因型分布经正和检验,除SE33等7个基因座外,均符合Hardy-Weinberg平衡定律。遗传学参数分析显示,41个基因座中SE33等21个属高鉴别力、高杂合度、高信息量,其余20个属中等高度多态性基因座。部分省份等位基因频率分布数据之间有显著性差异(P<0.05),各省与全国综合数据之间则无差异(P>0.05)。结论本文调查结果证实41个STR基因座均具有较强的个体识别能力,获得的数据可为法医学应用和筛选适合中国汉族人群基因座等研究和实践提供科学的基础数据。
Objective To investigate and analyze the genetic polymorphisms of 41 STR loci in 28 Chinese Han provinces and to provide a comprehensive and scientific basis for related research and identification. Methods A total of 9 126 unrelated individual blood samples were collected from 28 provinces / autonomous regions in Han Chinese. Forty-one STR loci were genotyped by Global FilerTM, AGCU EX-22 and AGCU 21 + 13 kit, and the distribution of alleles at each locus And genetic parameters, and the differences between the data test. Results Eight to 76 alleles were detected in 41 loci in 28 Chinese Han and Han nationality populations. The genotype distributions of each loci were tested by positive tests. Hardy-Weinberg equilibrium was found except for seven loci such as SE33. Genetic analysis showed that 21 of 41 loci were highly discriminatory, highly heterozygous and informative, and the remaining 20 were moderately high polymorphic loci. There was a significant difference (P <0.05) between the allele frequency distribution data in some provinces and the national comprehensive data (P> 0.05). Conclusion The results of this study confirmed that all 41 STR loci have strong individual identification ability. The data obtained can provide scientific and basic data for forensic applications and screening of loci for Chinese Han population.