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Objective:To investigate the potentials of the root bark of Annona(A.) senegalensis in the control of seizure and related hypnotic and motor incoordination effects in mice using experimental models.Methods:The methanol extract(ME) of the root bark of A.senegalensis was studied in mice using pentylenetetrazole(PTZ) induced convulsions,phenobarbitone induced sleeping time and motor coordination test on rota-rod performance.Acute toxicity and lethality(LD50) lest as well as phytochemical analysis were also carried out.Results:The extract(200,400,800 mg/ kg) exhibited a non- dose dependent significant(P<0.05) delay in the onset of both tonic and clonic phases of seizure induced by PTZ(60 mg/kg,s.c.) as well as offered a 100%protection (200 mg/kg) in mice from PTZ induced seizures.The extract significantly(P<0.05) decreased the latency and increased the duration of phenobarbitone induced sleeping time.At 200 mg/kg, the extract exhibited a significant(P<0.05) motor incoordination.The acute toxicity test revealed an oral LD_(50) of 1 2%mg/kg,while the phytochemical studies showed the presence of alkaloids, resins,glycosides,carbohydrate,reducing sugar,flavonoids,terpenoids,saponins and tannins. Conclusion:The extract of A senegalensis possessed anticonvulsant activity with pronounced hypnotic and muscle relaxant effects.
Objective: To investigate the potentials of the root bark of Annona (A.) Senegalensis in the control of seizure and related hypnotic and motor incoordination effects in mice using experimental models. Methods: The methanol extract (ME) of the root bark of A. senegalensis was studied in mice using pentylenetetrazole (PTZ) induced convulsions, phenobarbitone induced sleeping time and motor coordination test on rota-rod performance. Acute toxicity and lethality (LD50) lest as well as phytochemical analysis were also carried out. Results: The extract ( 200, 400, 800 mg / kg) delay in the onset of both tonic and clonic phases of seizure induced by PTZ (60 mg / kg, sc) as well as offered a 100% protection (200 mg / kg) in mice from PTZ induced seizures. The extract significantly (P <0.05) decreased the latency and increased the duration of phenobarbitone induced sleeping time. At 200 mg / kg, the extract showed a significant (P <0.05) motor incoordination The acute t oxicity test revealed an oral LD 50 (50) of 1 2% mg / kg while while the phytochemical studies showed the presence of alkaloids, resins, glycosides, carbohydrate, reducing sugar, flavonoids, terpenoids, saponins and tannins. Conclusion: The extract of A senegalensis possessed anticonvulsant activity with pronounced hypnotic and muscle relaxant effects.